• Elin Julianti School of Pharmacy, Bandung Institute of Technology (ITB)
  • Mochamad Fathurohman School of Pharmacy, Bandung Institute of Technology (ITB)
  • Sophi Damayanti School of Pharmacy, Bandung Institute of Technology
  • Rahmana Emran Kartasasmita School of Pharmacy, Bandung Institute of Technology (ITB)
Keywords: Biodiversity, Taxonomy, Marine resources


Docosahexaenoic acid (DHA) is one of essential fatty acids that are beneficial to health. Nowadays, the source of docosahexaenoic acid (DHA) is mainly obtained from fish which are extracted into fish oil products. However, the fish oil products still have some drawbacks in term of purity, acceptable flavor for costumers, and also their not environmental friendly production process. As an alternative solution, heterotrophic microalgae can be used as a potential source for DHA due to their excellence compared to fish oil products. The aim of this study is to isolate the heterotropic microalgae that can produce DHA. The heterotrophic microalgae were isolated from mangrove fallen leaves (Rhizophora apiculata) by using direct planting method. The morphology of pure microalgae colony were observed through light microscope and subsequently fermented for 14 days. Fatty acids were extracted and methylated through direct transesterification method. Identification and quantification of DHA were conducted by using gas chromatography. The results were four isolates of heterotropic microalgae, namely MTKC1, MTKC2, MTKC3, and MTKC4. The extract of MTKC2 that only showed the content of DHA with value of 9.2 % w/w. Therefore MTKC2 is a potential source for DHA. The MTKC2 was further identified by using molecular biology method and confirmed as Thraustochytrium aureum.


Download data is not yet available.


Abdel-Raouf, N., Al-Homaidan, A.A., Ibraheem, I.B.M. (2012). Microalgae and Waste Water Treatment. Saudi J Biol Sci., 19, 257-275

Bhadury, P., Mohammad, B.T., Wright, P.C. (2006). The current status of natural products from marine fungi and their potential as anti-infective agents. J. Ind. Microbiol. Biotechnol., 33, 325-337.

Bull, A.T., Stach, J.E.M. (2007). Marine actinobacteria: new opportunities for natural product search and discovery. Trends Microbiol., 15, 491–499

Batten, D., Peter C., Greg T. (2011). Resource Potential of Algae for Sustainable Biodiesel Production in the APEC. Presentation at APEC Workshop on Algal Biofuels San Francisco. 12-15.

Chisti, Y. (2007) Biodiesel from microalgae. Biotechnol. Adv., 25, 294-306

Dahmasa, I.F., Maroneb, P.A., Bauterb, M., Ryana A.S. (2011) Safety Evaluation of DHA-Rich Algal Oil from Schizochytrium sp., Food Chem Toxicol, 19(12), 3310-3318

Fenical, W, Jensen, P.R. (2006). Developing a new resource for drug discovery: marine actinomycete bacteria. Nat Chem Biol., 2(12), 666-73

Jeon, J.E., Julianti, E., Oh, H., Park, W., Oh, D-C., Oh, K-B., Shin, J. (2013). Stereochemistry of hydroxy-bearing benzolactones: Isolation and structural determination of chrysoarticulins A-C from a marine-derived fungus Chrysosporium articulatum. Tetrahedron Lett., 54, 3111-3115

Julianti, E., Oh, H., Jang, K.H, Lee, J.K, Lee, S.K., Oh, D.-C., Oh, K.-B., Shin, J. (2011). Acremostrictin, a Highly Oxygenated Metabolite from the Marine Fungus Acremonium strictum. J. Nat. Prod.,74, 2592-2594

Milne, J.L., Cameron, J.C., Page, L.E., Benson, S.M., Pakrasi, H.B. (2012). Algal Technologies for Biological Capture and Utilization of CO2 Require Breakthroughs in Basic Research. ACS Symposium Series, 1116, 107-141

Munoz, R., Guieysse, B. (2006). Algal-Bacterial Processes for the Treatment of Hazardous Contaminants: a review. Water Res., 40, 2799-2815

Newman, D.J. and Hill, R.T. (2006) New drugs from marine microbe: the tide is turning. J. Ind. Microbiol. Biotechnol., 33, 539-544.

Tan, L.T. (2007). Bioactive natural products from marine cyanobacteria for drug discovery. Phytochemistry, 68(7), 954-79.

Yang, H.L., Lu, C.K., Chen, S,F., Chen, Y.M., Chen, Y.M. (2010). Isolation and Characterization of Taiwanese Heterotrophic Microalgae: Screening of Strains for Docosahexaenoic Acid (DHA) Production. Mar. Biotechnol., 2, 85-173

Yokochi, T., Honda, D., Higashihara, T., Nakahara. T. (1998). Optimization of Docosahexaenoic Acid Production by S. Limacinum SR21. Appl Microbiol Biotechnol., 49, 72-76.

Wen, Z.Y., Chen, F. (2003). Heterotrophic Production of Eicosapentaenoic Acid by Microalgae. Biotechnol advance., 21, 273-294.

How to Cite
Julianti, E., Fathurohman, M., Damayanti, S., & Kartasasmita, R. (2018). ISOLATE OF HETEROTROPHIC MICROALGAE AS A POTENTIAL SOURCE FOR DOCOHEXAENOIC ACID (DHA). Marine Research in Indonesia, 43(2), 79-84.